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中南大学学报(英文版)

Journal of Central South University

Vol. 26    No. 2    February 2019

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Isolation of an acid producing Bacillus sp. EEEL02: Potential forbauxite residue neutralization
WU Hao(吴昊)1, LIAO Jia-xin(廖嘉欣)1, ZHU Feng(朱锋)1, Graeme MILLAR2, Ronan COURTNEY3, XUE Sheng-guo(薛生国)1

1. School of Metallurgy and Environment, Central South University, Changsha 410083, China;
2. Institute for Future Environments, Science and Engineering Faculty, Queensland University ofTechnology (QUT), Brisbane Qld 4000, Australia;
3. Department of Life Sciences, Schrodinger Building, University of Limerick, Co. Limerick, Ireland

Abstract:Bauxite residue deposit area (BRDA) is a typical abandoned mining wasteland representing extreme hostile environment with increased alkalinity. Microbially-driven neutralization of bauxite residue, based on the microbial acid producing metabolisms, is a novel strategy for achieving rapid pH neutralization and thus improving its environmental outcomes. The hypothesis was that these extreme conditions promote microbial communities which are capable of novel ecologically relevant functions. Several alkaliphilic acid producing bacteria were isolated in this study. One strain was selected for its superior growth pattern and acid metabolism (termed EEEL02). Based on the phylogenetic analysis, this strain was identified as Bacillus thuringiensis. The optimized fermentation conditions were as follows: pH 10; NaCl concentration 5%; temperature 25 °C; EEEL02 preferred glucose and peptone as carbon and nitrogen sources, respectively. Based on optimal fermentation conditions, EEEL02 induced a significant pH reduction from 10.26 to 5.62 in 5-day incubation test. Acetic acid, propionic acid and CO2 (g) were the major acid metabolites of fermentation, suggesting that the pH reduction in bauxite residue may be caused by acid neutralization derived from microbial metabolism. This finding provided the basis of a novel strategy for achieving rapid pH neutralization of bauxite residue.

 

Key words: bauxite residue; 16S rDNA; Bacillus thuringiensis; acid production; pH neutralization

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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