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中南大学学报(英文版)

Journal of Central South University

Vol. 27    No. 1    January 2020

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Characterization of acylated homoserine lactone derivatives and their influence on biofilms of Acidithiobacillus ferrooxidans BY-3 under arsenic stress
GAO Qi-yu(高启禹)1, 2, TANG De-ping(唐德平)1, SONG Peng(宋鹏)1, ZHOU Jian-ping(周剑平)3, LI Hong-yu(李红玉)1, 4

1. Institute of Microbiology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China;
2. Department of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China;
3. Institute of Biology, Gansu Provincial Academy of Sciences, Lanzhou 730000, China;
4. Institute of Microbiology and Biochemical Pharmacy, School of Pharmaceutics, Lanzhou University, Lanzhou 730000, China

Abstract:Quorum sensing is one kind of cell-to-cell signalling system among microorganisms that works in response to their population density via autoinducers exemplified by AHL and oligopeptides. In this study, fourteen AHL derivatives were synthesised by a chemical synthesis method, and two types of AHL derivatives were measured and screened by crystal violet staining assay, which have more obvious inhibitory effects on A. ferrooxidans biofilms under arsenic environment. Their structures were verified through IR and NMR identification. The morphological changes of A. ferrooxidans under the influence of the AHL derivatives were compared. In addition, the effects of AHL derivatives (0.1 μg/mL and 1 μg/mL) on membrane formation of A. ferrooxidans under high concentration of arsenic resistance (1,600 mg/L) were explored. Solid experimental data firstly showed that a portion of logarithmic microorganisms were ruptured under the effect of high arsenic concentration. Secondly, the volume of the cell shrank and the number of extracellular polymeric substances decreased after the addition of the AHL derivatives at high concentrations. Therefore, we found here that two derivatives used at concentrations of 0.1 μg/mL and 1 μg/mL accompanied with high concentration of arsenic can both effectively restrict biofilms formation by A. ferrooxidans.

 

Key words: acetylated homoserine lactone; chemical synthesis; Acidithiobacillus ferrooxidans; Quorum sensing; arsenic contamination

中南大学学报(自然科学版)
  ISSN 1672-7207
CN 43-1426/N
ZDXZAC
中南大学学报(英文版)
  ISSN 2095-2899
CN 43-1516/TB
JCSTFT
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